企業(yè)郵箱
搜索
科研活動(dòng)
論文名稱
A novel way of separation and preparation non-caffeine tea polyphenols from green tea waste
發(fā)表刊物
Advanced Materials Research
發(fā)表年度
2012
論文作者
譚和平、李懷平、宋航、徐文平、管馳、冉麗萍
承擔(dān)單位
生物所
收錄情況
關(guān)鍵詞
Noncaffeine,Preparation,Separation,Sephadex LH-20,Tea Polyphenol(TP)
摘要
A?simple,?clean?and?economical?way?of?separation?and?preparation?noncaffeine?tea?polyphenols?from?green?tea?waste?in?one-step?by?Sephadex?LH-20?column?chromatography?was?developed.?20%?(v/v)?ethanol?solution?was?used?for?removing?caffeine?and?other?components?in?the?first?stepwise?elution,?and?tea?polyphenols?was?eluted?with?60%?(v/v)?ethanol?solution?by?using?reversed?flow?in?the?second?stepwise?elution.?The?yield?of?tea?polyphenols?above?11%?with?the?purity?about?98%?and?the?content?of?catechins?more?than?91%,?and?the?content?of?caffeine?in?tea?polyphenols?have?not?been?detected?by?high-performance?liquid?chromatography.?The?recovery?of?tea?polyphenols?by?column?chromatography?were?about?86%,?and?the?stability?of?preparative?way?is?very?good.?Compared?with?conventional?extraction?and?decaffeination?ways,?this?way?has?more?excellent?advantages,?such?as?cheaper?raw?material,?no?toxic?residues,?higher?yield,?commercially?feasible?and?environment?friendly.
