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A novel way of separation and preparation non-caffeine tea polyphenols from green tea waste

Advanced Materials Research

2012

譚和平、李懷平、宋航、徐文平、管馳、冉麗萍

生物所

Noncaffeine,Preparation,Separation,Sephadex LH-20,Tea Polyphenol(TP)

A?simple,?clean?and?economical?way?of?separation?and?preparation?noncaffeine?tea?polyphenols?from?green?tea?waste?in?one-step?by?Sephadex?LH-20?column?chromatography?was?developed.?20%?(v/v)?ethanol?solution?was?used?for?removing?caffeine?and?other?components?in?the?first?stepwise?elution,?and?tea?polyphenols?was?eluted?with?60%?(v/v)?ethanol?solution?by?using?reversed?flow?in?the?second?stepwise?elution.?The?yield?of?tea?polyphenols?above?11%?with?the?purity?about?98%?and?the?content?of?catechins?more?than?91%,?and?the?content?of?caffeine?in?tea?polyphenols?have?not?been?detected?by?high-performance?liquid?chromatography.?The?recovery?of?tea?polyphenols?by?column?chromatography?were?about?86%,?and?the?stability?of?preparative?way?is?very?good.?Compared?with?conventional?extraction?and?decaffeination?ways,?this?way?has?more?excellent?advantages,?such?as?cheaper?raw?material,?no?toxic?residues,?higher?yield,?commercially?feasible?and?environment?friendly.